Coding

Part:BBa_K5043003

Designed by: Richard Meuten   Group: iGEM24_TUDarmstadt   (2024-08-17)
Revision as of 17:21, 17 August 2024 by Registry (Talk | contribs)

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phdE from Nocardioides sp. strain KP7

Encodes dihydrodiol dehydrogenase catalyzing reaction from Cis-4,5-Dihydro-4,5-dihydroxypyrene to 4,5-dihydroxypyrene. This is the second step in pyrene biodegradation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 658
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 658
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 329
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 658
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 658
    Illegal NgoMIV site found at 688
    Illegal NgoMIV site found at 793
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None