Coding

Part:BBa_K5043003

Designed by: Richard Meuten   Group: iGEM24_TUDarmstadt   (2024-08-17)


phdE from Nocardioides sp. strain KP7

Encodes dihydrodiol dehydrogenase presumably catalysing reaction from cis-4,5-Dihydro-4,5-dihydroxypyrene to 4,5-dihydroxypyrene. This is the second step in pyrene biodegradation. [1]

[1] S.-J. Kim, O. Kweon, R. C. Jones, J. P. Freeman, R. D. Edmondson, and C. E. Cerniglia, "Complete and integrated pyrene degradation pathway in Mycobacterium vanbaalenii PYR-1 based on systems biology," Journal of bacteriology, vol. 189, no. 2, pp. 464–472, 2007, doi: 10.1128/jb.01310-06

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 658
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 658
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 329
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 658
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 658
    Illegal NgoMIV site found at 688
    Illegal NgoMIV site found at 793
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None