Coding
Part:BBa_K5043003
Designed by: Richard Meuten Group: iGEM24_TUDarmstadt (2024-08-17)
phdE from Nocardioides sp. strain KP7
Encodes dihydrodiol dehydrogenase presumably catalysing reaction from cis-4,5-Dihydro-4,5-dihydroxypyrene to 4,5-dihydroxypyrene. This is the second step in pyrene biodegradation. [1]
[1] S.-J. Kim, O. Kweon, R. C. Jones, J. P. Freeman, R. D. Edmondson, and C. E. Cerniglia, "Complete and integrated pyrene degradation pathway in Mycobacterium vanbaalenii PYR-1 based on systems biology," Journal of bacteriology, vol. 189, no. 2, pp. 464–472, 2007, doi: 10.1128/jb.01310-06
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 658
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 658
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 329
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 658
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 658
Illegal NgoMIV site found at 688
Illegal NgoMIV site found at 793 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |