Regulatory

Part:BBa_K5267006:Design

Designed by: Pengyu Zheng   Group: iGEM24_NUDT-CHINA   (2024-08-17)
Revision as of 10:43, 17 August 2024 by Registry (Talk | contribs)

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6xCRE-Pmin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 118
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

You should consider the connection between the upstream and downstream sequence. The design was designed to account for the need to continuously accumulate the number of CRE copies, so two consecutive different restriction sites were added before the first CRE


Source

Homo Sapiens

References