Regulatory

Part:BBa_K5267006:Design

Designed by: Pengyu Zheng   Group: iGEM24_NUDT-CHINA   (2024-08-17)


6xCRE-Pmin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 118
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The connection between the upstream and downstream sequence is considered. Considering the need to continuously accumulate the number of CRE copies, two consecutive different restriction sites were added before the first CRE.

Source

Homo Sapiens

References