Coding
PCA

Part:BBa_K5291007:Design

Designed by: Rui Chen   Group: iGEM24_BNUZH-China   (2024-08-07)
Revision as of 09:59, 10 August 2024 by Kortybones (Talk | contribs) (Design Notes)


PAO102


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 153
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use it to improve the efficiency of carbon delivery between the two engineered bacteria. And we choose PAO102 because it natively exists in Pseudomonas aeruginosa PAO1 as well as its broad pH adjustion, which ensures its normal expression in the engineered P.aeruginosa.

Source

Pseudomonas aeruginosa PAO1

References

Lotlikar, S.R., et al., Three functional beta-carbonic anhydrases in Pseudomonas aeruginosa PAO1: role in survival in ambient air. Microbiology (Reading), 2013. 159(Pt 8): p. 1748-1759.