Part:BBa_K5291007:Design

PAO102

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 153
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Given that CO₂ is hard to trasnport in the biofilm formed by the two kinds of engineered bacteria, we use PAO102 to improve the efficiency of carbon delivery to avoid the harm of CO₂ release. And we choose this gene because it natively exists in Pseudomonas aeruginosa PAO1 as well as its broad pH adjustion, which ensures its normal expression in the engineered P.aeruginosa.

Source

Pseudomonas aeruginosa PAO1

References

[1]Lotlikar, S.R., et al., Three functional beta-carbonic anhydrases in Pseudomonas aeruginosa PAO1: role in survival in ambient air. Microbiology (Reading), 2013. 159(Pt 8): p. 1748-1759.
[2]Thorbecke, R., et al., The gene dosage effect of carbonic anhydrase on the biosynthesis of poly ( 3-hydroxybutyrate ) under autotrophic and mixotrophic culture conditions. POLYMER JOURNAL, 2021. 53(1): p. 209-213.