Part:BBa_K5226007

TD-glyA

Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1(parts:) and strengthened the endogenous C2 and C3 modules of Halomonas TD(parts:).

Usage and Biology

TD-GlyA is Serine hydroxymethyltransferase .The protein sequence is from Halomonas sp. TD01 strain.This protein catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.. This part is used in BBa_xx and BBa_xx.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 626
  Illegal PstI site found at 754
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 480
  Illegal PstI site found at 626
  Illegal PstI site found at 754
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 626
  Illegal PstI site found at 754
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 626
  Illegal PstI site found at 754
  Illegal AgeI site found at 985
  
• 1000
  COMPATIBLE WITH RFC[1000]