Part:BBa_K5226007

TD-glyA

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 626
  Illegal PstI site found at 754
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 480
  Illegal PstI site found at 626
  Illegal PstI site found at 754
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 626
  Illegal PstI site found at 754
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 626
  Illegal PstI site found at 754
  Illegal AgeI site found at 985
  
• 1000
  COMPATIBLE WITH RFC[1000]

Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1 and strengthened the endogenous C2 and C3 modules of Halomonas TD.

Usage and Biology

TD-GlyA is Serine hydroxymethyltransferase .The protein sequence is from Halomonas sp. TD01 strain.This protein catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.