Part:BBa_K5226006

TD-gcvP

Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1(parts:) and strengthened the endogenous C2 and C3 modules of Halomonas TD(parts:).

Usage and Biology

TD-GcvP is glycine dehydrogenase.The protein sequence is from Halomonas sp. TD01 strain.The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein during the glycine cleavage system(C2M) . This part is used in BBa_xx and BBa_xx.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 1564
  Illegal PstI site found at 1841
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1662
  Illegal SpeI site found at 1564
  Illegal PstI site found at 1841
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 1564
  Illegal PstI site found at 1841
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 1564
  Illegal PstI site found at 1841
  Illegal NgoMIV site found at 291
  Illegal NgoMIV site found at 1636
  
• 1000
  COMPATIBLE WITH RFC[1000]