Part:BBa_K5226006

TD-gcvP

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 1564
  Illegal PstI site found at 1841
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1662
  Illegal SpeI site found at 1564
  Illegal PstI site found at 1841
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 1564
  Illegal PstI site found at 1841
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 1564
  Illegal PstI site found at 1841
  Illegal NgoMIV site found at 291
  Illegal NgoMIV site found at 1636
  
• 1000
  COMPATIBLE WITH RFC[1000]

Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1 and strengthened the endogenous C2 and C3 modules of Halomonas TD.

Usage and Biology

TD-GcvP is glycine dehydrogenase.The protein sequence is from Halomonas sp. TD01 strain.The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein during the glycine cleavage system(C2M) .