Part:BBa_K5226002

AM1-mtdA

Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1(parts:) and strengthened the endogenous C2 and C3 modules of Halomonas TD(parts:).

Usage and Biology

AM1-MtdA is methylenetetrahydrofolate dehydrogenase. The protein sequence is from Methylobacterium extorquens AM1. This protein catalyzes the conversion of 5,10-methenyltetrahydrofolate into 5,10-methylene-5,6,7,8-tetrahydrofolate during the tetrahydrofolate (THF) cycle(C1M) . This part is used in BBa_xx and BBa_xx.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 94
  Illegal AgeI site found at 385
  
• 1000
  COMPATIBLE WITH RFC[1000]