Part:BBa_K5226002

AM1-mtdA

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 94
  Illegal AgeI site found at 385
  
• 1000
  COMPATIBLE WITH RFC[1000]

Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1 and strengthened the endogenous C2 and C3 modules of Halomonas TD.

Usage and Biology

AM1-MtdA is methylenetetrahydrofolate dehydrogenase. The protein sequence is from Methylobacterium extorquens AM1. This protein catalyzes the conversion of 5,10-methenyltetrahydrofolate into 5,10-methylene-5,6,7,8-tetrahydrofolate during the tetrahydrofolate (THF) cycle(C1M) .