Part:BBa_K4825049:Design

COX4-LEU4^S547∆

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 61
  Illegal XbaI site found at 871
  Illegal XbaI site found at 985
  Illegal SpeI site found at 1026
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 549
  Illegal SpeI site found at 1026
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1756
  Illegal BamHI site found at 702
  Illegal BamHI site found at 1668
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 61
  Illegal XbaI site found at 871
  Illegal XbaI site found at 985
  Illegal SpeI site found at 1026
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 61
  Illegal XbaI site found at 871
  Illegal XbaI site found at 985
  Illegal SpeI site found at 1026
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The 547th codon coding for S amino acid is deleted in order to diminish its sensitivity to leucine while maintainning its funciton since the presence of leucine can result in a decrease in the activity of LEU4. The composite part appends the first 26 amino acids of COX4 to the N-terminus of LEU4^S547∆ in order to relocate and overexpress LEU4^S547∆ in mitochondria.

Source

Saccharomyces cerevisiae

References

1. "ACS Synth. Biol. 2020, 9, 3, 546–555 Publication Date:February 12, 2020

https://doi.org/10.1021/acssynbio.9b00420"

2. DOI: 10.1021/acssynbio.9b00420