Coding

Part:BBa_K4825049:Design

Designed by: Ni Weizhao   Group: iGEM23_GreatBay-SCIE   (2023-10-11)


COX4-LEU4^S547∆


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 61
    Illegal XbaI site found at 871
    Illegal XbaI site found at 985
    Illegal SpeI site found at 1026
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 549
    Illegal SpeI site found at 1026
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1756
    Illegal BamHI site found at 702
    Illegal BamHI site found at 1668
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 61
    Illegal XbaI site found at 871
    Illegal XbaI site found at 985
    Illegal SpeI site found at 1026
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 61
    Illegal XbaI site found at 871
    Illegal XbaI site found at 985
    Illegal SpeI site found at 1026
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The 547th codon coding for S amino acid is deleted in order to diminish its sensitivity to leucine while maintainning its funciton since the presence of leucine can result in a decrease in the activity of LEU4. The composite part appends the first 26 amino acids of COX4 to the N-terminus of LEU4^S547∆ in order to relocate and overexpress LEU4^S547∆ in mitochondria.


Source

Saccharomyces cerevisiae

References

1. "ACS Synth. Biol. 2020, 9, 3, 546–555 Publication Date:February 12, 2020

https://doi.org/10.1021/acssynbio.9b00420"

2. DOI: 10.1021/acssynbio.9b00420