Composite

Part:BBa_K4759207

Designed by: Jianhua Chen   Group: iGEM23_Jiangnan-China   (2023-10-07)
Revision as of 07:37, 12 October 2023 by JianghuaChen (Talk | contribs)


T7-RBS4-Trx-linker-Olep

we fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 411
    Illegal AgeI site found at 459
    Illegal AgeI site found at 557
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None