Composite

Part:BBa_K4759207

Designed by: Jianhua Chen   Group: iGEM23_Jiangnan-China   (2023-10-07)


T7-RBS4-Trx-linker-Olep

Thioredoxin (Trx) is a series of oxidoreductases found in organisms that reduce disulfide binding by displacing thiodisulfide.
We add a solubilization-promoting tag at the N-teminal of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag.

protein-tags.png

Fig1: The application of protein tags


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 411
    Illegal AgeI site found at 459
    Illegal AgeI site found at 557
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None