Part:BBa_K2832180

BIOFAB Modular Promoter apFAB51

BBa_K2832180 is apFAB51 in the BIOFAB Collection . The BIOFAB Collection has constitutive promoters and transcription terminators. The collection is curated by the FSU iGEM teams . BBa_K2832180 (apFAB51) is an engineered constitutive promoter. Information about the design of this promoter is available in the BIOFAB Collectionpage. The FSU iGEM teams are characterizing all the parts in the BIOFAB Collection so they are useful to the iGEM community

Measurements

BBa_K2832180 (apFAB51) was used in the apFAB51 Measurement Device (BBa_K4680010). The biological device was inserted in the pSB1K3 plasmid backbone resulting in pFSUIGEM4. Figure 1 is the map of pFSUIGEM4. E. coli NEBExpress was transformed with pFSUIGEM4 resulting in the apFAB51 Test Cell.

apFAB51 Test Cell was grown in LB Broth medium then fluorescence was measured in a flow cytometer. The results of the measurements are depicted in Figure 2.

Figure 1: pFSUIGEM4 Map

Figure 1: pFSUIGEM4 Map

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]