Coding
Part:BBa_K4645018
Designed by: Meng Sun Group: iGEM23_HZAU-China (2023-10-11)
A gene that encodes a response regulatory protein
When the extracellular concentration of CSP increases to a certain threshold, CSP binds to the extracellular domain of the histidine kinase receptor encoded by comD on the cell membrane. And when the histidine kinase receptor is phosphorylated at aspartate to activate the intracellular response regulator protein comE,the phosphorylated comE protein binds to specific downstream gene promoters and regulates the expression of downstream target genes regulated by bacterial density sensing.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1143
Illegal PstI site found at 185 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1143
Illegal PstI site found at 185 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1143
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1143
Illegal PstI site found at 185 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1143
Illegal PstI site found at 185 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 981
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Categories
Parameters
None |