Coding

Part:BBa_K4645018

Designed by: Meng Sun   Group: iGEM23_HZAU-China   (2023-10-11)


A gene that encodes a response regulatory protein

When the extracellular concentration of CSP increases to a certain threshold, CSP binds to the extracellular domain of the histidine kinase receptor encoded by comD on the cell membrane. And when the histidine kinase receptor is phosphorylated at aspartate to activate the intracellular response regulator protein ComE, the phosphorylated ComE protein binds to specific downstream gene promoters and regulates the expression of downstream target genes regulated by bacterial density sensing.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1143
    Illegal PstI site found at 185
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1143
    Illegal PstI site found at 185
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1143
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1143
    Illegal PstI site found at 185
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1143
    Illegal PstI site found at 185
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 981


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Categories
Parameters
None