Coding

Part:BBa_K4604006:Design

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-09-30)
Revision as of 12:30, 10 October 2023 by NikitaEdel (Talk | contribs) (→‎Design Notes)


bluB mutated


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Change of start codon to adjust to E. coli. Additional site directed mutagenesis to result in a stop codon. No Methyonine triplet close to the inserted stop codon, translation should certainly end abruptly.

Source

Taken from Mycobacterium Smegmatis, synthesized by IDT.

References