Coding

Part:BBa_K4604006:Design

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-09-30)


bluB mutated


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Change of start codon to adjust to E. coli. Additional site directed mutagenesis to result in a stop codon. No Methyonine triplet close to the inserted stop codon, translation should certainly end abruptly.

Source

Taken from Mycobacterium Smegmatis, sequence was taken from the National Library of Medicine (gene ID:61603312), synthesized by IDT.