Coding

Part:BBa_K187027:Design

Designed by: Julia Pon   Group: iGEM09_Alberta   (2009-10-19)
Revision as of 17:17, 19 October 2009 by JuliaPon (Talk | contribs) (→‎Design Notes)

rplC, 50S ribsomal subunit, L3 in pBA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 256
    Illegal XbaI site found at 10
    Illegal PstI site found at 646
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 256
    Illegal PstI site found at 646
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 256
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 256
    Illegal XbaI site found at 10
    Illegal PstI site found at 646
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 256
    Illegal XbaI site found at 10
    Illegal PstI site found at 646
    Illegal AgeI site found at 169
    Illegal AgeI site found at 604
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note that the rplC gene in this plasmid starts at the start codon and ends at the stop codon. pBA does not include a promoter or terminator. pBA does include an RBS consensus sequence positioned 8 bp upstream of the ATG. We recommend that to express rplC, you use the Biobytes assembly method together with the promoter and terminator parts we have submited in pAB and pBA to assemble promoters and terminators onto rplC. As there is a range of promoters to chose from, this allows rapid manipulation of gene expression level. Using the Biobytes method, several DNA segments can be combined in just 20min per segment.

Source

k

References