Part:BBa_K187027:Design
rplC, 50S ribsomal subunit, L3 in pBA
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 256
Illegal XbaI site found at 10
Illegal PstI site found at 646 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 256
Illegal PstI site found at 646 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 256
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 256
Illegal XbaI site found at 10
Illegal PstI site found at 646 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 256
Illegal XbaI site found at 10
Illegal PstI site found at 646
Illegal AgeI site found at 169
Illegal AgeI site found at 604 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Note that the rplC gene in this plasmid starts at the start codon and ends at the stop codon. pBA does not include a promoter or terminator. pBA does include an RBS consensus sequence positioned 8 bp upstream of the ATG. We recommend that to express rplC, you use the Biobytes assembly method together with the promoter and terminator parts we have submited in pAB and pBA to assemble promoters and terminators onto rplC. As there is a range of promoters to chose from, this allows rapid manipulation of gene expression level. Using the Biobytes method, several DNA segments can be combined in just 20min per segment.
Source
MG1655 E.coli genomic DNA was the template for rplC amplification. Plasmid pBA is entered as part K187001.