Regulatory
Part:BBa_K177047
Designed by: Michael Lower Group: iGEM09_Warsaw (2009-10-18)
crobox alternative version
Derivative of OR3
This variant of the cro binding box differs from the OR1 (BBa_K177002) version by binding strength to Cro and λ repressor, as indicated by the free energy change, being -1.1 kcal/mol and 2.2 kcal/mol respectively.1
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
1 Takeda Y., Sarai A., Rivera VM.; Analysis of the sequence-specific interactions between Cro repressor and operator DNA bt systematic base substitution experiments; Proc. Natl. Acad. Sci. USA, Vol. 86, pp. 439-443, January 1989
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Categories
Parameters
None |