Part:BBa_K177047
crobox alternative version
Derivative of OR3
Cro-box is the particular fragment of phage lambda DNA which is recognized by cro repressor protein. This sequence is artificially designed (based on natural fragment of lambda phage genome) to regulate the affinity of the cro protein.
Usage and Biology
When this part is introduced into a sequence of elements expressed from a single promoter the binding of the Cro protein (BBa_K177009) successfully inhibits the expression of elements located downstream.
Functional Parameters
This variant of the cro binding box differs from the OR1 (BBa_K177002) version by binding strength to Cro and λ repressor, as indicated by the free energy change, being -1.1 kcal/mol and 2.2 kcal/mol respectively.1
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
1 Takeda Y., Sarai A., Rivera VM.; Analysis of the sequence-specific interactions between Cro repressor and operator DNA bt systematic base substitution experiments; Proc. Natl. Acad. Sci. USA, Vol. 86, pp. 439-443, January 1989
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