Part:BBa_K190019:Design

fMT

fMT is a metallothionein, binding Arsenite(III) and Arsenate(V), it has higher affinity for As(III).

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 38
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 127
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Add BioBrick prefix and suffix, also a RBS (BBa_B0034) will be added.
In working with heavy metal accumulation considerations should be made in the labwork required and also in the obtaining higher concentrations of the heavy metals in bacteria.

Source

fMT was cloned from pMAL-MT (kindly provided by Wilfred Chen, University of California), the origin of the gene is the genome of Fucus vesiculosus.

[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=nucleotide&dopt=GenBank&RID=AJPJ1GVY016&log%24=nuclalign&blast_rank=1&list_uids=3819155 GenBank: AJ005834.1]

References

Gold B, Deng H, et al(2008). "[http://www.ncbi.nlm.nih.gov/pubmed/18724363 Identification of a copper-binding metallothionein in pathogenic mycobacteria]". Nat Chem Biol. 4(10): 609-616

Klaassen, Curtis D., Supratim Choudhuri, James M. McKim, Jr., Lois D. Lehman-McKeeman, and William C. Kershaw (1994). [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567434/ "In Vitro and In Vivo Studies on the Degradation of Metallothionein"]. Environ. Health Perspect 102(3): 141-146.

Shailendra Singh, Ashok Mulchandani, Wilfred Chen (February 2008). "[http://dx.doi.org/10.1128/AEM.02871-07 Highly Selective and Rapid Arsenic Removal by Metabolically Engineered Escherichia coli Cells Expressing Fucus vesiculosus Metallothionein]". MICROBIOLOGY 74(9): 2924–2927