Regulatory

Part:BBa_K4613097

Designed by: Qinfei Zhou   Group: iGEM23_NAU-CHINA   (2023-10-09)
Revision as of 17:44, 9 October 2023 by QinfeiZhou (Talk | contribs) (→‎Reference)


Plac

IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. This part additionally includes the lac operator(symmetric), which was optimized for tight binding of lac repressor to avoid the leakage of the downstream gene.

Reference

  1. White CE, Winans SC. Identification of amino acid residues of the Agrobacterium tumefaciens quorum-sensing regulator TraR that are critical for positive control of transcription. Mol Microbiol. 2005 Mar;55(5):1473-86. doi: 10.1111/j.1365-2958.2004.04482.x. PMID: 15720554.
  2. Fuqua C, Winans SC. Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes. J Bacteriol. 1996 Jan;178(2):435-40. doi: 10.1128/jb.178.2.435-440.1996. PMID: 8550463; PMCID: PMC177675.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 60
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 60
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 60
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 60
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 60
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None