Regulatory

Part:BBa_K4613097

Designed by: Qinfei Zhou   Group: iGEM23_NAU-CHINA   (2023-10-09)


Plac

IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. This part additionally includes the lac operator(symmetric), which was optimized for tight binding of lac repressor to avoid the leakage of the downstream gene.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 60
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 60
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 60
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 60
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 60
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None