![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4800038
Designed by: Chenxi Ma Group: iGEM23_NJTech-China-B (2023-10-08)
pTrc99a-Hsd36
3beta-hydroxy-DELTA5-steroid dehydrogenase from Oenococcus alcoholitolerans. The conversion is carried out in two separate, sequential reactions; in the first reaction, which requires NAD+, the enzyme catalyses the dehydrogenation of the 3beta-hydroxy steroid to a 3-oxo intermediate. In the second reaction the reduced coenzyme, which remains attached to the enzyme, activates the isomerization of the Delta5 form to a Delta4 form. Substrates include dehydroepiandrosterone (which is converted into androst-5-ene-3,17-dione), pregnenolone (converted to progesterone) and cholest-5-en-3-one, an intermediate of cholesterol degradation.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 3353
Illegal PstI site found at 3365 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 3365
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3347
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 3353
Illegal PstI site found at 3365 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 3353
Illegal PstI site found at 3365
Illegal AgeI site found at 2611
Illegal AgeI site found at 2683
Illegal AgeI site found at 3292 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3549
Illegal BsaI site found at 4617
Illegal SapI.rc site found at 767
[edit]
Categories
Parameters
None |