Composite

Part:BBa_K4800038

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-08)


pTrc99a-Hsd36

We linearized the pTrc99a vector using restriction endonuclease and connected the target gene Hsd36 to it through homologous recombination to obtain the recombinant plasmid pTrc99a-Hsd36.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3353
    Illegal PstI site found at 3365
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3365
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3347
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3353
    Illegal PstI site found at 3365
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3353
    Illegal PstI site found at 3365
    Illegal AgeI site found at 2611
    Illegal AgeI site found at 2683
    Illegal AgeI site found at 3292
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3549
    Illegal BsaI site found at 4617
    Illegal SapI.rc site found at 767


[edit]
Categories
Parameters
None