Coding
SazCA

Part:BBa_K4665120:Design

Designed by: Fien Eickmans   Group: iGEM23_MSP-Maastricht   (2023-09-28)
Revision as of 13:47, 8 October 2023 by FienEick (Talk | contribs) (→‎Design Notes)


SazCA Carbonic Anhydrase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Coding sequence for the thermostable α-carbonic anhydrase SazCA which catalyzes the reversible hydration reaction of


This sequence will be attached to a flexible linker sequence at its 5' end for the final construct of our fusion protein

Source

This part is derived from the genome of extremophilic bacterium Sulfurihydrogenibium azorense. Our sequence is derived from the paper by Zhu et al., (2022), which codon-optimized for the SazCA sequence for E. coli BL21(DE3).

References

Zhu, Y., et.al. (December 6, 2021). Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralisation. Synthetic and Systems biotechnology, 7(1): 460-473. https://doi.org/10.1016%2Fj.synbio.2021.11.008