Coding
SazCA

Part:BBa_K4665120:Design

Designed by: Fien Eickmans   Group: iGEM23_MSP-Maastricht   (2023-09-28)


SazCA Carbonic Anhydrase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence will be attached to a flexible linker sequence at its 5' end for the final construct of our fusion protein

Source

This part is derived from the genome of extremophilic bacterium Sulfurihydrogenibium azorense.

Our sequence for SazCa is derived from the paper by Zhu et al. (2022), which has been codon-optimized for E. coli BL21(DE3).

References

Zhu, Y., et.al. (December 6, 2021). Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralisation. Synthetic and Systems biotechnology, 7(1): 460-473. https://doi.org/10.1016%2Fj.synbio.2021.11.008