![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4800051
Designed by: Qiming Zou Group: iGEM23_NJTech-China-B (2023-10-08)
pTrc99a-YahK-SpuC
To compare the activity of different transaminases, the DNA fragments of Ptrc-RBS-YahK were amplified and ligated into the plasmids of pTrc99a-SpuC.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 4846
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 4846
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3624
Illegal XhoI site found at 5140 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 4846
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 4846
Illegal AgeI site found at 4129
Illegal AgeI site found at 4582 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5346
Illegal BsaI site found at 6414
Illegal SapI.rc site found at 767
[edit]
Categories
Parameters
None |