Composite

Part:BBa_K4800051

Designed by: Qiming Zou   Group: iGEM23_NJTech-China-B   (2023-10-08)


pTrc99a-YahK-SpuC

We linearized the pTrc99a-YahK vector using restriction endonuclease and connected the target gene SpuC to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK-SpuC.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 4846
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 4846
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3624
    Illegal XhoI site found at 5140
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 4846
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 4846
    Illegal AgeI site found at 4129
    Illegal AgeI site found at 4582
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5346
    Illegal BsaI site found at 6414
    Illegal SapI.rc site found at 767


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Categories
Parameters
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