Composite

Part:BBa_K190034:Design

Designed by: Michael Verhoeven   Group: iGEM09_Groningen   (2009-09-07)
Revision as of 18:54, 18 October 2009 by PaulSchavemaker (Talk | contribs) (→‎Source)

GVP with ZntR regulated promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 411
    Illegal BamHI site found at 4216
    Illegal XhoI site found at 3904
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 862
    Illegal NgoMIV site found at 1264
    Illegal AgeI site found at 4183
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2674
    Illegal SapI.rc site found at 3830


Design Notes

Melbourne GVP cluster was used containing 400bp repeat in gvpL


Source

E.coli and Bacillus megaterium

References