Composite

Part:BBa_K190034:Design

Designed by: Michael Verhoeven   Group: iGEM09_Groningen   (2009-09-07)

GVP with ZntR regulated promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 411
    Illegal BamHI site found at 4216
    Illegal XhoI site found at 3904
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 862
    Illegal NgoMIV site found at 1264
    Illegal AgeI site found at 4183
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2674
    Illegal SapI.rc site found at 3830


Design Notes

Melbourne GVP cluster was used containing the 400bp repeat in gvpL.

Source

E.coli and Bacillus megaterium

References