DNA
stgRNA 1

Part:BBa_K4630000

Designed by: Zhejun Qin   Group: iGEM23_WHU-China   (2023-10-06)
Revision as of 05:14, 6 October 2023 by Registry (Talk | contribs)

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self-targeting guide RNA 1

This composite part consists of a Cas9 coding sequence (BBa_K1218011), and a Lambda-Red recombinases coding sequence (BBa_K1433005). The expression of Cas9 and Lambda-Red is under the control of araBAD promoter. We demonstrated that this one plasmid editing system can perform both genome and plasmid editing, with high editing efficiency and no mutations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1642
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3921
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 6510
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4863
    Illegal BsaI.rc site found at 4840


[edit]
Categories
//function/crispr
//function/crispr/grna
Parameters
None