DNA
stgRNA 1

Part:BBa_K4630000

Designed by: Zhejun Qin   Group: iGEM23_WHU-China   (2023-10-06)


self-targeting guide RNA 1

The stgRNA 1 serves for self-targeting with high efficiency. First, we designed the SDS using our own N20 generator (see Model). Also, the sgRNA scaffold right after the SDS sequence was modified to "GGG", which gives a PAM for the knock-out.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1642
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3921
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 6510
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4863
    Illegal BsaI.rc site found at 4840


[edit]
Categories
//function/crispr
//function/crispr/grna
Parameters
None