Plasmid_Backbone

Part:pSB1AC3:Experience

Designed by: Tom Knight   Group: iGEM05_Registry_Example   (2005-06-07)
Revision as of 17:04, 17 October 2009 by Verhoeven1981 (Talk | contribs) (→‎User Reviews)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of pSB1AC3

User Reviews

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Antiquity

This review comes from the old result system and indicates that this part worked in some test.

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Aberdeen_Scotland 2009

The transformation, miniprep and the gel (undigested and digested with the EcoRI and PstI) worked. Further testings were performed by the formation of the BBa_K182001 and BBa_K182005, PCR analysis, sequencing and everything worked as expected.

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iGEM Groningen 2009

The transformations with pSB1AC3 (containing different biobricks of own design) into E. coli TOP10 cells, growth on both antibiotics, and gel analysis (undigested and digested with the EcoRI and PstI) worked as expected. The high (copy) number of plasmids per cell make it an easy to work with plasmid, ideal for cloning and assembly work.

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