Composite

Part:BBa_K4227025

Designed by: Yang Chen   Group: iGEM22_ZJUT-China   (2022-10-13)
Revision as of 17:43, 13 October 2022 by Kaga (Talk | contribs)


PnlmC-muB-sigma28

The CSP is the component stimulating peptides of the S.mutans. When there is CSP be sensed by the comE, the comE will be phosphorylated and start the PnlmC promoter, and the muB will be expressed to decompose the EPS

σs have been divided into four groups based on their phylogenetic relationships and modular structure. σ28, a Group 3 σ, is the most widely distributed alternative σ factor, making it an attractive candidate for study of its promoter recognition. σ28 controls expression of flagella-related genes in all motile Gram-negative and Gram-positive bacteria, and plays a role in development in some non-motile bacteria. We use the σ28 to start the P28 promotor

The muB is a kind of dextransucrase that used to decompose the Extracellular Polymeric Substances (EPS) of the Streptococcus mutans<i></p> Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 669
    Illegal EcoRI site found at 1638
    Illegal EcoRI site found at 3173
    Illegal PstI site found at 1587
    Illegal PstI site found at 1888
    Illegal PstI site found at 2200
    Illegal PstI site found at 2763
    Illegal PstI site found at 3487
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 669
    Illegal EcoRI site found at 1638
    Illegal EcoRI site found at 3173
    Illegal NheI site found at 1113
    Illegal PstI site found at 1587
    Illegal PstI site found at 1888
    Illegal PstI site found at 2200
    Illegal PstI site found at 2763
    Illegal PstI site found at 3487
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 669
    Illegal EcoRI site found at 1638
    Illegal EcoRI site found at 3173
    Illegal BglII site found at 413
    Illegal BglII site found at 676
    Illegal BglII site found at 2974
    Illegal BglII site found at 3746
    Illegal BamHI site found at 3355
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 669
    Illegal EcoRI site found at 1638
    Illegal EcoRI site found at 3173
    Illegal PstI site found at 1587
    Illegal PstI site found at 1888
    Illegal PstI site found at 2200
    Illegal PstI site found at 2763
    Illegal PstI site found at 3487
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 669
    Illegal EcoRI site found at 1638
    Illegal EcoRI site found at 3173
    Illegal PstI site found at 1587
    Illegal PstI site found at 1888
    Illegal PstI site found at 2200
    Illegal PstI site found at 2763
    Illegal PstI site found at 3487
    Illegal NgoMIV site found at 801
    Illegal NgoMIV site found at 1703
    Illegal NgoMIV site found at 2702
    Illegal NgoMIV site found at 2991
    Illegal NgoMIV site found at 3069
    Illegal NgoMIV site found at 3543
    Illegal NgoMIV site found at 3888
    Illegal AgeI site found at 257
  • 1000
    COMPATIBLE WITH RFC[1000]

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