Part:BBa_K4227025

P_nlmC-muB-sigma28

Usage and Biology

The CSP is the component stimulating peptides of the S.mutans. When there is CSP be sensed by the comE, the comE will be phosphorylated and start the P_nlmC promoter, and the muB will be expressed to decompose the EPS^[1]

σs have been divided into four groups based on their phylogenetic relationships and modular structure. σ28, a Group 3 σ, is the most widely distributed alternative σ factor, making it an attractive candidate for study of its promoter recognition. σ28 controls expression of flagella-related genes in all motile Gram-negative and Gram-positive bacteria and plays a role in development in some non-motile bacteria. We use the σ28 to start the P₂₈ promotor^[2]

The muB is a kind of dextransucrase that used to decompose the Extracellular Polymeric Substances (EPS) of the Streptococcus mutans^[3]

Characterization

(The following experimental data are from the literature and modeling, not the actual data made by the experiment)

Since our experiment is severely influenced by COVID-19 in experimenter, materials, equipment and so on this year. We have to collect these data by the literature and modeling

Our modeling was based on the law of mass action.

Fig 1. The expression level of the muB.

Reference

[1] Tianlei Liu, Shoubin Xue, Wenbo Cai, Xiaojing Liu, Xiaojuan Liu, Rongrong Zheng, Hongyan Luo, Wenhui Qi. ComCED signal loop precisely regulates nlmC expression in Streptococcus mutans. Annals of Microbiology.2013;1 (64) : 31-38..

[2] R Lutz. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Research.1997;6 (25) : 1203-1210.

[3] Nobuyuki Sumitomo, Katsuhisa Saeki, Katsuya Ozaki, Susumu Ito, Tohru Kobayashi. Mutanase from a Paenibacillus isolate: Nucleotide sequence of the gene and properties of recombinant enzymes. Biochimica et Biophysica Acta (BBA) - General Subjects.2007;4 (1770) : 716-724. Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 669
  Illegal EcoRI site found at 1638
  Illegal EcoRI site found at 3173
  Illegal PstI site found at 1587
  Illegal PstI site found at 1888
  Illegal PstI site found at 2200
  Illegal PstI site found at 2763
  Illegal PstI site found at 3487
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 669
  Illegal EcoRI site found at 1638
  Illegal EcoRI site found at 3173
  Illegal NheI site found at 1113
  Illegal PstI site found at 1587
  Illegal PstI site found at 1888
  Illegal PstI site found at 2200
  Illegal PstI site found at 2763
  Illegal PstI site found at 3487
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 669
  Illegal EcoRI site found at 1638
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  Illegal BglII site found at 413
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  Illegal BglII site found at 3746
  Illegal BamHI site found at 3355
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 669
  Illegal EcoRI site found at 1638
  Illegal EcoRI site found at 3173
  Illegal PstI site found at 1587
  Illegal PstI site found at 1888
  Illegal PstI site found at 2200
  Illegal PstI site found at 2763
  Illegal PstI site found at 3487
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 669
  Illegal EcoRI site found at 1638
  Illegal EcoRI site found at 3173
  Illegal PstI site found at 1587
  Illegal PstI site found at 1888
  Illegal PstI site found at 2200
  Illegal PstI site found at 2763
  Illegal PstI site found at 3487
  Illegal NgoMIV site found at 801
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  Illegal NgoMIV site found at 2702
  Illegal NgoMIV site found at 2991
  Illegal NgoMIV site found at 3069
  Illegal NgoMIV site found at 3543
  Illegal NgoMIV site found at 3888
  Illegal AgeI site found at 257
  
• 1000
  COMPATIBLE WITH RFC[1000]