Coding

Part:BBa_K4439009

Designed by: Pauline Verchinine   Group: iGEM22_EPFL   (2022-09-29)
Revision as of 14:59, 12 October 2022 by Charlottedml (Talk | contribs)

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GST-N[AS]8C-CBD-10xHis

Abstract

The GST-N[AS]8C-CBD-10xHis protein was implemented in a pET28a vector. This protein could allow to test the polymerisation of [AS] modules (Part:BBa K3944000) present in recombinant green lacewing silk proteins N[AS]8C (Part:BBa K4439002) to create a very specific binding between two proteins.

The assembly compatibility of this part surprised the team: we think that we don't have any assembly compatibility because of the fact that we did a small mutation on the CBD sequence that is not taken into account in the part we used (Part:BBa K1321014).

The mutation is at bp153 where a C was changed into a T, which is a silent mutation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2592
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2592
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2592
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2592
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2592
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 82
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Categories
Parameters
None