Regulatory

Part:BBa_K3944000

Designed by: Olof Dahlman   Group: iGEM21_Chalmers-Gothenburg   (2021-09-14)


ScCUP1p

The copper-based induction system (CUP1) is native to yeast where it responds to copper ions by producing metallothioneins and help to protect the cell from toxic levels. Yeast naturally expresses ACE1, a copper-dependent transcription factor. The transcription factor activates by binding to copper ions, and in turn activates the CUP1 promoter containing four metal regulatory elements. By inserting CUP1 upstream from a desired gene we can use the same system to stimulate expression. Since the system is native to yeast, no additional associated proteins need be included to make it work.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None