Part:BBa_K4438707

P4G13_trigger_4_phi29

P4G13_trigger_4_phi29(BBa_K4438707) is single-stranded DNA having 15 nucleotides. Figure 1B) shows the secondary structure and minimum free energy.

Usage and Biology

The part P4G13_trigger_4_phi29(BBa_K4438707) has complete complementarity to the part P4G13_aptamer (BBa_K4438700) and blocks a part of the binding domain of the aptamer. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. Progesterone binds with P4G13_aptamer (BBa_K4438700) with high affinity and displaces the P4G13_trigger_4_phi29(BBa_K4438707) [1]. This part has complete complementarity with part P4G13_Target_4 (BBa_K4438708). Phi 29 DNA extension polymerase extends the template strand and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. Different levels of progesterone can be detected using all these three parts.

https://parts.igem.org/File:T--IISER-Tirupati_India--Pg413_4.png

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Contreras Jiménez, G., Eissa, S., Ng, A., Alhadrami, H., Zourob, M., & Siaj, M. (2015). Aptamer-based label-free impedimetric biosensor for detection of progesterone. Analytical chemistry, 87(2), 1075-1082.