Part:BBa_K4438700

P4G13_aptamer

P4G13_aptamer is a single-stranded DNA aptamer having 60 nucleotides. Figure 1A) shows the secondary structure and minimum free energy.

Usage and Biology

This part with KD of 17 nM (calculated using electrochemical impedance spectroscopy (EIS) and fluorometric assay) has high specificity for progesterone. No cross-reactivity has been detected to analogues of progesterone like 17β-estradiol (E2) and norethisterone (NET). Nucleotides 27-58 from the 5’ end form the binding domain [3]. It has been found that when this aptamer was hybridised with a complementary sequence of 10 nucleotides, the aptamer showed a significant conformation change. This part can be used to develop various electrochemical and fluorescence biosensors. It has applications in food safety, environmental analysis and clinical studies [1-3]. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1].Contreras Jiménez, G., Eissa, S., Ng, A., Alhadrami, H., Zourob, M., & Siaj, M. (2015). Aptamer-based label-free impedimetric biosensor for detection of progesterone. Analytical chemistry, 87(2), 1075-1082. [2].Cui, H., Lu, H., Yang, J., Fu, Y., Huang, Y., Li, L., & Ding, Y. (2022). A Significant Fluorescent Aptamer Sensor Based on Carbon Dots and Graphene Oxide for Highly Selective Detection of Progesterone. Journal of Fluorescence, 32(3), 927-936. [3].Alhadrami, H. A., Chinnappan, R., Eissa, S., Rahamn, A. A., & Zourob, M. (2017). High affinity truncated DNA aptamers for the development of fluorescence based progesterone biosensors. Analytical biochemistry, 525, 78-84.