Part:BBa_K4388004

Vitis vinifera Resveratrol O-Methyltransferase Mutant (VvROMT S29P)

The Vitis vinifera resveratrol o-methyltransferase (VvROMT) is an enzyme that catalyses the dimethylation of resveratrol to form pterostilbene.

ROMT is a key enzyme used for the production of pinostilbene or pterostilbene. This version of the enzyme is a mutant with amino acid change S29P. This mutations has been shown to increase catalytic efficiency from 128 (wild-type) to 158 μmol/h/g (Yan et al., 2021).

The ORF of this compound was obtained from NCBI GenBank code: FM178870.1. This sequence was then codon optimized for E. coli K12, mutated at the desired positions, and two stop codons (TAATAA) were added to terminate translation.

This part has been successfully ligated into a transcriptional unit: https://parts.igem.org/Part:BBa_K4388008:Experience

Yan, Z.-B., Liang, J.-L., Niu, F.-X., Shen, Y.-P., & Liu, J.-Z. (2021). Enhanced Production of Pterostilbene in Escherichia coli Through Directed Evolution and Host Strain Engineering. Frontiers in Microbiology, 12. doi:10.1186/s12934-017-0644-6

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 116
  Illegal BamHI site found at 366
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]