Part:BBa_K4388008:Experience

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KCL iGEM 2022

Through JUMP type IIS Level 1, this transcriptional unit (BBa_K4388008) was assembled in a one-pot digestion reaction with BsaI and ligation into pJUMP29-1D. Successful ligation and transformation were selected for by identifying non-fluorescent colonies on kanamycin agar plates (Fig. 1).

Figure 1. Construction of Transcriptional Unit (TU) BBa_K4388008 into Level 1 pJUMP29-1D vector. Level 0 parts BBa_K4388011, BBa_K4388004, and BBa_B0015 were assembled into the transcriptional unit BBa_K4388008 in pJUMP29-1D via a one-pot BsaI digestion reaction. White (sfGPF-excised) colonies indicate successful ligation of the TU into plasmid, and successful transformation of plasmid conferring kanamycin resistance. Final level 1 plasmid constructs were designed using SnapGene.

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