Part:BBa_K4339002

MaSp1_K3264003mod_CT E. australis

A major ampullate spidroin 1 (MaSp1) based protein consisting of the Great Bay 2019 part BBa_K3264003 fused to a C-terminal domain from the African nursery web spider Euprosthenops australis. Due to the highly repetitive nature of spidroin proteins, the DNA coding sequences are also highly repetitive. Unfortunately we were unable to submit the original Great Bay sequence for synthesis, and therefore K3264003 had to be modified to allow for synthesis.

Usage and Biology

Sequence and Features

The sequence originates from the African nursery web spider Euprosthenops australis [1].

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

All MaSp parts (K4339002 - K4339010) were designed to be integrated into devices, fused with solubility tag TrxA (BBa_K3619001) with a thrombin cleavage site between them. TrxA is a small, highly soluble protein which can act as a molecular chaperon, guiding protein folding and preventing inclusion body formation

References

[1] - Stark M et al. Macroscopic fibers self-assembled from recombinant miniature spider silk proteins. 2007;8(5): 1695–1701. doi: https://doi.org/10.1021/bm070049y.

[2] - LaVallie E et al. Thioredoxin as a fusion partner for production of soluble recombinant proteins in Escherichia coli. Methods in Enzymology. 2000;326: 322-340. doi: https://doi.org/10.1016/S0076-6879(00)26063-1