Part:BBa_K3619001
cDNA encoding the protein thioredoxin 1.
The TrxA gene is a protein coding sequence translated to the protein thioredoxin 1, or Trx1. It is a 109 aa long protein from the protein group thioredoxins which are present in almost all living organisms. They play an important role in oxidative stress responses and in redox signaling[1].
Thioredoxins are thermally stable, highly soluble and have solid folding characteristics. There are small, highly expressed, and have both N- and C-terminals exposed, making them a good choice for acting as a fusion partner [2].
Trx1 have been used as a fusion protein for improved expression of small peptides. It may increase solubility and expression rate, aid in refolding of target protein, make purification easier and can later be removed from the peptide by protease cleavage. [3]
For more information of the protein, visit the Uniprot record P0AA25 [4].
Sources:
1. https://doi.org/10.1016/B978-0-12-370873-1.00019-8
2. https://doi.org/10.1016/S0076-6879(00)26063-1
3. https://doi.org/10.1016/j.pep.2017.08.003
4. https://www.uniprot.org/uniprot/P0AA25
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |