Coding

Part:BBa_K4234000

Designed by: Robert Kaptur   Group: iGEM22_Duke   (2022-10-11)
Revision as of 02:59, 12 October 2022 by Aq14 (Talk | contribs)


IDH1 R132H mutation

This part contains the coding region of IDH1 R132H mutation. IDH1 codes for isocitrate dehydrogenase 1 catalyzing the oxidative decarboxylation of isocitrate to 2-oxoglutarate. This reaction is thought to be essential in control of oxidative damage in the cell. R132H variant of IDH1 is known to be prevalent in gliomas and glioblastomas.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 253
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 253
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 253
    Illegal BglII site found at 11
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 253
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 253
    Illegal NgoMIV site found at 1105
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 970
    Illegal BsaI.rc site found at 1093
    Illegal SapI.rc site found at 777


[edit]
Categories
Parameters
None